For complete folding and serine protease activity NS3 necessitates the intercalation of a present in NS4A(Refs 21 NS4A is as little(54 protein) Protein that anchors NS3 to cellular membranes using an N terminal hydrophobic peptide24. Proper folding of the serine protease domain also requires sychronisation of Zn2 by three cysteine residues distal from the active site.
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